Overview

Filter Cubes for BX3 and IX3 Microscopes

Download the transmittance wavelength data for U-F Series

Unit Name Excitation Filter Emission Filter Dichromatic Mirror
Ultraviolet Excitations U-FUW 340-390 420IF 410
U-FUN 360-370 420IF 410
U-FUNA 360-370 420-460 410
Blue Violet Excitations U-FBVW 400-440 460IF 455
Blue Excitations U-FBW 460-495 510IF 505
U-FBWA 460-495 510-550 505
U-FBN 470-495 510IF 505
U-FBNA 470-495 510-550 505
Green Excitations U-FGW 530-550 575IF 570
U-FGWA 530-550 575-625 570
U-FGNA 540-550 575-625 570
Yellow Excitations U-FYW 540-585 600IF 595
Fluorescent Protein Variants U-FCFP 425-445CFP 460-510CFP 455CFP
U-FGFP 460-480GFP 495-540GFP 490GFP
U-FYFP 490-500YFP 515-560YFP 515YFP
U-FRFP 535-555HQ 570-625HQ 565HQ
U-FMCHE 565-585 600-690 595

Filter Cubes for IX3 Microscopes

Unit Name Excitation Filter Emission Filter Dichromatic Mirror
Ultraviolet Excitations IX3-FUWXL 340-390 420IF 410
Blue Violet Excitations IX3-FBVWXL 400-440 460IF 455
Green Excitations IX3-FGWXL 530-550 575IF 570
Fluorescent Protein Variants IX3-FCFPXL 425-445CFP 460-510CFP 455CFP
IX3-FGFPXL 460-480GFP 495-540GFP 490GFP
IX3-FYFPXL 490-500YFP 515-560YFP 515YFP
IX3-FMCHEXL 565-585 600-690 595

Filter Cubes for IX2, BX2, and MVX10 Microscopes

Download the transmittance wavelength data for U-M Series

Unit Name Excitation Filter Emission Filter Dichromatic Mirror
Ultraviolet Excitations U-MWU2 330-385 420 400
U-MNU2 360-370 420 400
U-MNUA2 360-370 420-460 400
Violet Excitations U-MNV2 400-410 455 455
Blue Violet Excitations U-MWBV2 400-440 475 455
U-MNBV2 420-440 475 455
Blue Excitations U-MSWB2 420-480 520IF 500
U-MWB2 460-490 520IF 500
U-MNB2 470-490 520IF 500
U-MWIB3 460-495 510IF 505
U-MWIBA3 460-495 510-550 505
U-MNIB3 470-495 510IF 505
U-MNIBA3 470-495 510-550 505
Green Excitations U-MWG2 510-550 590 570
U-MNG2 530-550 590 570
U-MWIG3 530-550 575IF 570
U-MWIGA3 530-550 575-625 570
U-MNIGA3 540-550 575-625 570
Yellow Excitations U-MWIY2 545-580 610IF 600
Fluorescent Protein Variants U-MCFPHQ 425-445HQ 460-510HQ 450
U-MGFPHQ 460-480HQ 495-540HQ 485
U-MYFPHQ 490-500HQ 515-560HQ 505
U-MRFPHQ 535-555HQ 570-625HQ 565

Selection Chart

Fluorescent Protein Variants

Excitation Maximum (nm) Emission Maximum (nm) Applicable BX3/IX3 Mirror Unit Applicable BX2WI/MVX10 Mirror Unit
AmCyan 458 489 U-FCFP, U-FBVW U-MWBV2, U-MNBV2, U-MCFPHQ
Azami-Green 492 505 U-FBWA, U-FBNA, U-FGFP, U-FBW, U-FBN U-MWIB3, U-MWIBA3, U-MNIB3, U-MNIBA3, U-MGFPHQ
DsRed2 563 582 U-FGW, U-FGWA, U-FGNA, U-FRFP U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ
EBFP 380 440 U-FUNA, U-FUW, U-FUN U-MWU2, U-MNU2, U-MNUA2, U-MNV2
ECFP 433 475 U-FCFP, U-FBVW U-MCFPHQ, U-MWBV2, U-MNBV2, U-MNV2
EGFP 488 507 U-FBWA, U-FBNA, U-FGFP, U-FBW, U-FBN U-MGFPHQ, U-MNIB3, U-MWIBA3, U-MWIB3, U-MNIBA3
EYFP 513 527 U-FBWA, U-FBNA, U-FYFP, U-FBW, U-FBN U-MYFPHQ, U-MWIBA3, U-MNIBA3, U-MWIBA3, U-MNIBA3, U-MNIB3, U-MNB2, U-MWB2, U-MWIB3
Fucci (mAG1) 492/551 505/565 U-FBNA, U-FGFP, U-FBWA, U-FBW, U-FBN U-MNIBA3, U-MGFPHQ, U-MNIB3, U-MWIBA3, U-MWIB3
GFP (S65T) 488 510 U-FBWA, U-FBNA, U-FGFP, U-FBW, U-FBN U-MGFPHQ, U-MWIBA3, U-MNIBA3, U-MWIB3, U-MNIB3
GFP (WT) 395, 475 510 U-FBWA, U-FBNA, U-FGFP, U-FBW, U-FBN U-MWIBA3, U-MNIBA3, U-MGFPHQ, U-MWIB3, U-MNIB
HcRed 588 618 U-FYW U-MWIY2
Midoriishi-Cyan 472 495 U-FBWA, U-FBW, U-FBNA, U-FBN, U-FCFP, U-FGFP U-MWIBA3, U-MWIB3, U-MNIBA3, U-MNIB3, U-MCFPHQ, U-MGFPHQ
mCherry 580 610 U-FMCHE, U-FYW U-MWIY2
mRFP 584 607 U-FMCHE, U-FYW U-MWIY2
mStrawberry 574 596 U-FMCHE, U-FYW U-MWIY2
RFP (DsRed) 558 583 U-FRFP, U-FGW, U-FGWA, U-FGNA U-MRFPHQ, U-MWIG3, U-MWIGA3, U-MNIGA3
Venus 515 528 U-FYFP U-MYFPHQ, U-MNB2, U-MNIB3, U-MNIBA3, U-MSWB2, U-MWB2, U-MWIB3, U-MWIBA3

Photoactivation

Excitation Maximum (nm) Emission Maximum (nm) Applicable BX3/IX3 Mirror Unit Applicable BX2WI/MVX10 Mirror Unit
Dronpa-Green 503 518 U-FBN, U-FBNA, U-FBW, U-FBWA, U-FYFP U-MGFPHQ, U-MNB2, U-MNIB3, U-MNIBA3, U-MWB2, U-MWIB3, U-MWIBA3, U-MYFPHQ
PA-GFP 504 517 U-FGFP, U-FBWA, U-FBNA, U-FBW, U-FBN U-MGFPHQ, U-MNIB3, U-MWIBA3, U-MWIB3, U-MNIBA3

Photoconversion

Excitation Maximum (nm) Emission Maximum (nm) Applicable BX3/IX3 Mirror Unit Applicable BX2WI/MVX10 Mirror Unit
Kaede (Green/Red) 509/573 518/582 U-FBN, U-FBNA, U-FBW, U-FBWA, U-FGFP, U-FYFP, U-FGW, U-FGWA, U-FGNA, U-FRFP U-MWIB3, U-MWIBA3, U-MGFPHQ, U-MYFPHQ, U-MNB2, U-MNIB3, U-MNIBA3,U-MWB2, U-MNIGA3, U-MRFPHQ, U-MWIG3, U-MWIGA3

Carbohydrate Modification, Protein Modification (Labeling)

Protein modification

Excitation Maximum (nm) Emission Maximum (nm) Applicable BX3/IX3 Mirror Unit Applicable BX2WI/MVX10 Mirror Unit
Alexa Fluor 350 346 442 U-FUNA, U-FUW, U-FUN U-MNUA2, U-MWU2, U-MNU2
Alexa Fluor 405 402 421 U-FUNA, U-FUW, U-FUN U-MNUA2, U-MWU2, U-MNU2
Alexa Fluor 430 434 539 U-FBVW U-MWBV2, U-MNBV2, U-MNV2
Alexa Fluor 488 495 519 U-FBWA, U-FBNA, U-FBW, U-FBN U-MWIBA3, U-MNIBA3, U-MWIB3, U-MNIB3,UMNB2, U-MWB2
Alexa Fluor 532 531 554 U-FGW, U-FGWA, U-FGNA, U-FRFP U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ
Alexa Fluor 546 556 573 U-FGW, U-FGWA, U-FGNA, U-FRFP U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ
Alexa Fluor 555 555 567 U-FGW, U-FGWA, U-FGNA, U-FRFP U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ
Alexa Fluor 568 578 603 U-FGW, U-FGWA, U-FGNA, U-FRFP, U-FYW, U-MCHE U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ, U-MWIY2
Alexa Fluor 594 590 617 U-FYW, U-FMCHE U-MWIY2
Allophycocyanin (APC) 650 660 U-FYW U-MWIY2
7-amino-4-methylcoumarin-3-acetic acid (AMCA) 349 448 U-FUNA, U-FUW, U-FUN U-MNUA2, U-MWU2, U-MNU2
BODIPY FL 505 513 U-FBWA, U-FBW, U-FBNA, U-FBN, U-FGFP U-MWIBA3, U-MWIB3, U-MNIBA3, U-MNIB3
Cascade Blue 376 425 U-FUNA, U-FUW, U-FUN U-MNUA2, U-MWU2, U-MNU2
Fluorescein-isothiocyanate (FITC) 494 518 U-FBWA, U-FBW, U-FBNA, U-FBN, U-FGFP, U-FYFP U-MWIBA3, U-MNIBA3, U-MWB2, U-MNB2, U-MWIB3, U-MNIB3, U-MGFPHQ, U-MYFPHQ
Oregon Green 488 496 524 U-FBWA, U-FBW, U-FBNA, U-FBN, U-FGFP, U-FYFP U-MWIBA3, U-MNIBA3, U-MWB2, U-MNB2, U-MWIB3, U-MNIB3, U-MGFPHQ, U-MYFPHQ
Phycoerythrin B (PE-B) 545 576 U-FGW, U-FGWA, U-FGNA, U-FRFP U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ
Phycoerythrin R (PE-R) 490, 565 578 U-FGNA, U-FGW, U-FGWA, U-FRFP U-MNIGA3, U-MWIG3, U-MWIGA3, U-MRFPHQ
Rhodamine B-isothiocyanate (RITC) 543 580 U-FGW, U-FGWA, U-FGNA, U-FRFP U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ
Texas Red 595 615 U-FYW, U-MCHE U-MWIY2
Tetramethylrhodamine-isothyocyanate (TRITC) 541 572 U-FGW, U-FGWA, U-FGNA, U-FRFP U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ
1-Anilinonaphthalene-8-sulfonic acid (ANS) 365 460 U-FUW, U-FUN U-MWU2, U-MNU2
CPM (7-Diethylamino-3-(4′-Maleimidylphenyl)-4-Methylcoumarin) ~384 470 U-FUW, U-FUN U-MWU2, U-MNU2
Cy2 489 506 U-FBWA, U-FBNA, U-FBW, U-FBN U-MWIBA3, U-MNIBA3, U-MWIB3, U-MNIB3
Cy3 (512); 550 570; (615) U-FGW, U-FGWA, U-FGNA U-MWIG3, U-MWIGA3, U-MNIGA3
Cy3.5 581 594; (640) U-FYW, U-FMCHE U-MWIY2
Dans (1-Dimethyl Amino Naphaline 5 Sulphonic Acid) 340 525 U-FUW, U-FUN U-MWU2, U-MNU2
Dansyl chloraide 360 520 U-FUW, U-FUN U-MWU2, U-MNU2
Dansyl hydrazine 340 525 U-FUW, U-FUN U-MWU2, U-MNU2
Eosin 525 545 U-FBWA, U-FBNA, U-FBW, U-FBN U-MWIBA3, U-MNIBA3, U-MWIB3, U-MNIB3
Fluorescamine 390 465/475 U-FUW, U-FUN, U-FNUA U-MWU2, U-MNU2, U-MNUA2, U-MNV2
FluorX 494 520 U-FBWA, U-FBNA, U-FBW, U-FBN U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MSWB2, U-MWB2, U-MNB2
Lucifer Yellow CH 425 528 U-FBVW, U-FBW, U-FBW, U-FBWA U-MWBV2, U-MNBV2, U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MWB2, U-MNIB2, U-MSWB2
Monochlorobimane (mBCI) 394 490 U-FUW, U-FUN, U-FUNA U-MNUA2, U-MWU2, U-MNU2, U-MNV2
o-phtalaldehyde (OPT) 340 455 U-FUNA, U-FUW, U-FUN U-MNUA2, U-MWU2, U-MNU2
Resorufin 572 583 U-FGW, U-FGWA, U-FGNA U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWG2, U-MNG2
SITS (Primuline) 395-425 450 U-FUW, U-FUN, U-FUNA U-MNUA2, U-MWU2, U-MNU2, U-MNV2
SITS (Stilbene Isothiosulphonic acid) 365 460 U-FUW, U-FUN, U-FUNA U-MNUA2, U-MWU2, U-MNU2

Carbohydrate modification

Excitation Maximum (nm) Emission Maximum (nm) Applicable BX3/IX3 Mirror Unit Applicable BX2WI/MVX10 Mirror Unit
Acrifl avine 436 520 U-FBVW, U-FBW U-MWBV2, U-MNBV2, U-MWB2, U-MSWB2

Nuclei, Nucleic Acid Staining

Nuclei, nucleic acid

Excitation Maximum (nm) Emission Maximum (nm) Applicable BX3/IX3 Mirror Unit Applicable BX2WI/MVX10 Mirror Unit
Acridine Orange 500 530/630-650 U-FBW, U-FBN, U-FBWA, U-FBNA, U-FYFP U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Acridine Yellow 470 550 U-FBW, U-FBN, U-FBWA, U-FBNA U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MWB2, U-MNIB2, U-MSWB2
Acrifl avine-Feulgen 455 515 U-FBVW, U-FBW, U-FBWA U-MWBV2, U-MNBV2, U-MWIB3, U-MWIBA3, U-MWB2, U-MSWB2
Auramine O-Feulgen 460 550 U-FBW, U-FBWA, U-FBNA U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MWB2, U-MNIB2, U-MSWB2
7-Amino Actinomycin D 546 647 U-FYW, U-FGW, U-FMCHE U-MWIG3, U-MWG2, U-MNG2, U-MWIY2
bis-Aminophenyl-oxadizole (BAO) -Feulgen 380 470 U-FUW, U-FUN, U-FUNA U-MWU2, U-MNU2, U-MNUA2
BOBO 1 462 481 U-FBVW U-MWBV2, U-MNBV2, U-MCFPHQ
BO PRO 1 462 481 U-FBVW U-MWBV2, U-MNBV2
Chromomycin A3 445 575 U-FBVW U-MWBV2, U-MNBV2, U-MSWB2
4, 6-diamidino-2-phenyl-indole HCI (DAPI) 345 455 U-FUW, U-FUN, U-FUNA U-MWU2, U-MNU2, U-MNUA2
Ethidium Bromide 493 620 U-FGW, U-FGWA, U-FGNA U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWG2, U-MNG2, U-SWG2
Hoechst 33258 345 478 U-FUW, U-FUN, U-FUNA U-MWU2, U-MNU2, U-MNUA2
Hoechst 33342 343 483 U-FUW, U-FUN, U-FUNA U-MWU2, U-MNU2, U-MNUA2
Mithramycin 445 575 U-FBVW U-MWBV2, U-MNBV2, U-MSWB2
Olivomycin 430 545 U-FBVW U-MWBV2, U-MNBV2
Pararosaniline-Feulgen 570 625 U-FYW, U-FMCHE, U-FGW, U-FGWA, U-FGNA U-MWIY2, U-MWG2, U-MNG2, U-MWIG3, U-MWIGA3, U-MNIGA3
POPO 1 434 456 U-FBVW, U-FCFP U-MNV2, U-MWBV2, U-MNBV2, U-MCFPHQ
Propidium Iodide (PI) 536 617 U-FGW, U-FGWA, U-FGNA, U-FYW U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWIY2
Pyronin Y 540 570 U-FGW, U-FGWA, U-FGNA U-MWIG3, U-MWIGA3, U-MNIGA3
Quinacrine Mustard 423 503 U-FBVW U-MWBV2, U-MNBV2
SYTOX Green nucleic acid stain 504 523 U-FBW, U-FBN, U-FBWA, U-FBNA U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
Thiazole Orange 510 530 U-FBW, U-FBN, U-FBWA, U-FBNA U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
TOTO 1 509 533 U-FBW, U-FBN, U-FBWA, U-FBNA U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3
TOTO 3 642 661 U-FYW, U-FMCHE U-MWIY2
TO PRO 3 642 661 U-FYW, U-FMCHE<