Overview
Simultaneous Multicolor TIRF
The Olympus cellTIRF system provides true simultaneous acquisition of up to four wavelengths.
- Motorized angle control for each laser provides equal evanescence (±1 nm) penetration, enabling high-contrast images of cell surface and single molecules and minimizing background noise
- cellTIRF-4Line system has integrated point FRAP optics for the first laser line, enabling FRAP and photo-switching experiments with no additional costs
Imaging Stability
The frame architecture and focus drive design of the IXplore system offer enhanced rigidity that reduces the impact of vibration and temperature. It maintains desired positions along the X-, Y-, and Z-axes to facilitate reliable time-lapse and multipoint imaging. When combined with the Olympus ultrasonic stage (IX3-SSU) and TruFocus system, the IXplore TIRF microscope system can capture high-precision, multipoint time-lapse images that are aligned and in focus.
TIRF Objectives
Total internal reflection fluorescence (TIRF) is facilitated by a wide range of objectives featuring a high signal-to-noise ratio and a correction collar to adjust for cover glass thickness and temperature. Our corrected plan apochromat objectives with an NA of 1.5 help you acquire uniform high-quality images with a large field of view. Take advantage of Olympus’ remarkable TIRF objective with the world’s highest NA, 1.7*.
*As of November, 2018. According to Olympus research.
Precise and Intuitive Photomanipulation (Optional Peripherals)
Advanced Analysis
Advanced functionality, such as object tracking, Count and Measure, Kymograph and real time 2D deconvolution to enhance object identification.
Fast, Microsecond-Accurate Devices
The fast filter wheel, shutter, LED light source control, and real-time controllers (U-RTC) enable less photobleaching and phototoxicity, resulting in healthier cells and more robust data.
Specifications
| Microscope Frame | IX83P2ZF | ||
|---|---|---|---|
| Observation Method | Total Internal Reflection Fluorescence | ✓ | |
| Fluorescence (Blue/Green Excitation) | ✓ | ||
| Fluorescence (Ultraviolet Excitation) | ✓ | ||
| Differential Interference Contrast (DIC) | ✓ | ||
| Phase Contrast | ✓ | ||
| Brightfield | ✓ | ||
| Revolving Nosepiece | Motorized (6 position) | ✓ | |
| Focus | Motorized | ✓ | |
| Z Drift Compensator | ✓ | ||
| Observation Tubes | Widefield (FN 22) | Tilting Binocular | ✓ |
| Illuminator | Transmitted Köhler Illuminator | LED Lamp | ✓ |
| 100 W Halogen Lamp | ✓ | ||
| Fluorescence Illuminator | 100 W Mercury Lamp | ✓ | |
| Light Guide Illumination | ✓ | ||
| Fluorescence Mirror Turret | Motorized (8 position) | ✓ | |
| Stage | Motorized | IX3-SSU Ultrasonic Stage for IX3 |
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| Mechanical | IX3-SVR Mechanical Stage with Right Handle |
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| IX3-SVL Mechanical Stage with Left Short Handle |
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| Condenser | Motorized | Universal Condenser | W.D. 27 mm, NA 0.55, motorized aperture and polarizer |
| Manual | Universal Condenser | NA 0.55/ W.D. 27 mm | |
| Ultra-Long Working Distance Condenser | NA 0.3/ W.D. 73.3 mm | ||
| Confocal Scanner | – | ||
| Super Resolution Processing | – | ||
| Accessories | Remote correction collar controller (IX3-RCC) Real time controller (U-RTC/U-RTCE) TIRF illuminator Incubation housing | ||
| Dimensions (W × D × H) | 323 (W) x 475 (D) x 706 (H) mm (IX83 microscope frame) | ||
| Weight | Approx. 47kg (IX83P2ZF) | ||