Simultaneous Multicolor TIRF

The Olympus cellTIRF system provides true simultaneous acquisition of up to four wavelengths.

  • Motorized angle control for each laser provides equal evanescence (±1 nm) penetration, enabling high-contrast images of cell surface and single molecules and minimizing background noise
  • cellTIRF-4Line system has integrated point FRAP optics for the first laser line, enabling FRAP and photo-switching experiments with no additional costs

Learn more about the cellTIRF system

Imaging Stability

The frame architecture and focus drive design of the IXplore system offer enhanced rigidity that reduces the impact of vibration and temperature. It maintains desired positions along the X-, Y-, and Z-axes to facilitate reliable time-lapse and multipoint imaging. When combined with the Olympus ultrasonic stage (IX3-SSU) and TruFocus system, the IXplore TIRF microscope system can capture high-precision, multipoint time-lapse images that are aligned and in focus.

TIRF Objectives

Total internal reflection fluorescence (TIRF)  is facilitated by a wide range of objectives featuring a high signal-to-noise ratio and a correction collar to adjust for cover glass thickness and temperature. Our corrected plan apochromat objectives with an NA of 1.5 help you acquire uniform high-quality images with a large field of view. Take advantage of Olympus’ remarkable TIRF objective with the world’s highest NA, 1.7*.

*As of November, 2018. According to Olympus research. 

Learn more about our high NA objectives

Precise and Intuitive Photomanipulation (Optional Peripherals)

Olympus’ cellFRAP photomanipulation device and real-time controller (U-RTCE) enable accurate control (200 µs dead time), diffraction-limited stimulation with a flexible region of interest, and precise reproduction of experimental conditions.

Learn more about the cellFRAP

Advanced Analysis

Advanced functionality, such as object tracking, Count and Measure, Kymograph and real time 2D deconvolution to enhance object identification.

Fast, Microsecond-Accurate Devices

The fast filter wheel, shutter, LED light source control, and real-time controllers (U-RTC) enable less photobleaching and phototoxicity, resulting in healthier cells and more robust data.

Learn more about the U-RTC/E


Microscope Frame IX83P2ZF
Observation Method Total Internal Reflection Fluorescence
Fluorescence (Blue/Green Excitation)
Fluorescence (Ultraviolet Excitation)
Differential Interference Contrast (DIC)
Phase Contrast
Revolving Nosepiece Motorized (6 position)
Focus Motorized
Z Drift Compensator
Observation Tubes Widefield (FN 22) Tilting Binocular
Illuminator Transmitted Köhler Illuminator LED Lamp
100 W Halogen Lamp
Fluorescence Illuminator 100 W Mercury Lamp
Light Guide Illumination
Fluorescence Mirror Turret Motorized (8 position)
Stage Motorized IX3-SSU Ultrasonic Stage for IX3
  • X: 114 mm, Y: 75 mm
Mechanical IX3-SVR Mechanical Stage with Right Handle
  • X: 114 mm, Y: 75 mm
IX3-SVL Mechanical Stage with Left Short Handle
  • X: 114 mm, Y: 75 mm
Condenser Motorized Universal Condenser W.D. 27 mm, NA 0.55, motorized aperture and polarizer
Manual Universal Condenser NA 0.55/ W.D. 27 mm
Ultra-Long Working Distance Condenser NA 0.3/ W.D. 73.3 mm
Confocal Scanner
Super Resolution Processing
Accessories Remote correction collar controller (IX3-RCC) Real time controller (U-RTC/U-RTCE) TIRF illuminator Incubation housing
Dimensions (W × D × H) 323 (W) x 475 (D) x 706 (H) mm (IX83 microscope frame)
Weight Approx. 47kg (IX83P2ZF)