Resolve confocal images down to 120 nm XY resolution using the confocal technique and Olympus super resolution (OSR).

Learn more about the Olympus super resolution

*Image: Stress fibers of Hela cell: Antibody staining with Phalloidin-Alexa488 (green) for actin, Alexa 568 (red) for myosin heavy chain.
Image data courtesy of: Keiju Kamijo,Ph.D. Division of Anatomy and Cell Biology, Faculty of Medicine, TOHOKU Medical and Pharmaceutical University

OSR algorithms work in real time to eliminate delays caused by frame averaging or image reconstruction, providing instant super resolution images so you can get to your results faster.

This enables experimental design for super resolution to include live cell experiments, which are further improved through the ultrafast imaging speeds and multichannel acquisition capabilities of the spinning disk confocal.

EB3 proteins binding to the top of microtubles extending in HeLa live cells. EB3 proteins were GFP- labeled by means of transgenesis.

_{Image data courtesy of: Kaoru Kato, PhD, National Institute of Adovanced Industrial Science and Technology Biomedical Research Institute}

*Click on images above for zoom view

Easily integrate the IXplore SpinSR microscope system into existing experiment and sample protocols; you can switch from widefield, confocal, and super resolution using the same samples with just the click of a button— the microscope takes care of the rest.

Image data can be further enhanced using cellSens software’s image analysis tools. The software’s efficient workflows enable users to effectively manage their data and perform advanced analysis that helps unlock new insights.

TruSight deconvolution algorithms are designed to work seamlessly with OSR algorithms, helping prevent over processing. Together they provide sharper, clearer images than either technique alone.

To observe intracellular structures, it is necessary to prevent out of focus fluorescence from blurring the true data in your final image. The IXplore SpinSR microscope system has incorporated a confocal optical system, enabling the use of various objectives, including silicone oil optics, therefore allowing the acquisition of sharp super resolution images with less blurring even in thick samples. Furthermore, no special fluorophores or imaging buffers are required so there is no need to change samples or preparation protocols when looking to convert to super resolution.

Time-lapse image of mouse primary neuron labeled with EGFP after co-culture with astrocyte for 2 weeks. Easy to see the difference between immature spine (yellow arrow) and mature spine (blue arrow), and detect the morphological change in time. 3D was acquired with exposure time 500 ms/frame, 0.15 um Z step for 41 slices. Images were acquired every 2 minutes for 1 hour. 3D displayed by FV31S-DT.

_{Image data courtesy of: Yuji Ikegaya, PhD
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo}

Our TruSight deconvolution can be combined with super resolution to provide images that clearer and sharper than with deconvolution alone. The 3D constrained iterative deconvolution removes blur in the Z-axis for a cleaner three-dimensional image.

• High-speed image processing with the advanced TruSight deconvolution
• TruSight deconvolution is compatible with Olympus Super Resolution

*Image: Mouse kidney tissue stained with Alexa488

The IXplore SpinSR system can use two cameras simultaneously to achieve fast, two-color localization super-resolution imaging. This is achieved using existing fluorophores and laser lines.

Mitotic spindle at metapahse cell

_{HeLa cells derived from human cervical cancer were fixed and stained for α-tublin(microtubules,red) and Hec1(kinetochores, green),respectively. DNA was stained with DAPI(chromosomes,blue). Chromosomes interact with microtubules constituting mitotic spindle via kinetochores assembled on centromere region of chromosomes.
Image courtesy of: Masanori Ikeda and Kozo Tanaka, Department of molecular oncology,Institute of Development, Aging and Cancer}

Nuclear pore complex of Hela cell

_{Nup153（Alexa 488: green), Nup62(Alexa 555: red)
Image courtesy of: Hidetaka Kosako, Fujii Memorial Institute of Medical Sciences, Tokushima university}

References

S. Hayashi and Y. Okada, “Ultrafast superresolution fluorescence imaging with spinning disk confocal microscope optics,” Mol. Biol. Cell 26(9), 1743–1751 (2015).

S. Hayashi, “Resolution doubling using confocal microscopy via analogy with structured illumination microscopy,” Jpn. J. Appl. Phys. 55(8), 082501 (2016).

A. Nagasawa-Masuda and K. Terai, “Yap/Taz transcriptional activity is essential for vascular regression via Ctgf expression and actin polymerization,” PLoS ONE 12(4), e0174633 (2017).

H. Nakajima, et al., “Flow-Dependent Endothelial YAP Regulation Contributes to Vessel Maintenance,” Dev. Cell 40(6), 523-536.e6 (2017).

K. Tateishi, et al., “Three-dimensional Organization of Layered Apical Cytoskeletal Networks Associated with Mouse Airway Tissue Development,” Sci. Rep. 7, 43783 (2017).

E. Herawati, et al., “Multiciliated cell basal bodies align in stereotypical patterns coordinated by the apical cytoskeleton,” J. Cell Biol. 214(5) 571-586 (2016).

M.-T. Ke, et al., “Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing Agent,” Cell Rep. 14(11) 2718–2732 (2016).

Olympus Super Resolution (OSR) technology realizes lateral (XY) resolution down to 120 nm. It is uniquely designed to take advantage of high spatial frequency data in confocal images. After processing, the final images are not only sharper through a reduction in point size but also better resolved for structures very close together.

Learn more about the OSR technology