Overview
High-Sensitivity, High-Resolution Deep Multiphoton Imaging
The FVMPE-RS multiphoton microscope employs advanced technology and optical design to enhance sensitivity and resolution during deep imaging.
- Broad 400 nm to 1600 nm spectral transmission window efficiently delivers near-infrared excitation without compromising short wavelength detection
- Large-area detection path collects more emission signal, especially large-angle scattered photons
- TruResolution objectives offer automated spherical aberration compensation to increase brightness and resolution, revealing fine details at every plane within a deep image stack
High-Speed Imaging for Fast, Dynamic Cellular Processes
High-speed resonant scanning and high-resolution linear scanning are standard.
- Full frame imaging at 30 frames-per second (fps) reduces motion artifacts during in vivo and live cell imaging
- 438 fps maximum imaging speed enables you to capture rapid, dynamic phenomena, such cell transport during blood flow and calcium signaling events in neurons and other cells
Multiwavelength Excitation for Broader Spectral Coverage
The FVMPE-RS imaging platform supports a dual wavelength infrared pulsed laser or two independent tunable infrared lasers for multichannel, multiphoton excitation imaging.
- Optimally excite different fluorophores without having to repeatedly tune the laser
- Independent power control of each laser line makes it easier to simultaneously capture fluorophores with differing efficiencies
- Laser wavelengths beyond 1000 nm enables access to the growing library of red-shifted fluorescent dyes and proteins and practical third-harmonic generation imaging
Third harmonic generation imaging of porcine adipose tissue. Unlabeled porcine adipose tissue was irradiated with femtosecond laser at 1250 nm, second harmonic is detected from collagen fibers at 625 nm and third harmonic from lipid interfaces at 416 nm.
Optional Features for Advanced Applications
The FVMPE-RS multiphoton microscope is a modular platform, enabling you to easily upgrade your system as your research needs grow.
Options include:
- An independent scanner path for optogenetics and other photomanipulation experiments
- Analog input channels to support electrophysiology experiments
- Digital TTL input/output lines to coordinate sensory stimuli with multiphoton imaging during behavioral experiments
Intuitive Software Optimized for Multiphoton Observation
The customizable software layout gives you more flexibility, increasing your efficiency:
- Tailor the UI to your experiments
- Choose to see only the tools you need for fast access to advanced features
- Save acquisition parameters from each experiment and recall them for repeatable and consistent imaging
Online analysis and image processing, including spectral unmixing and 3D rendering, come standard.
3 Microscope Frame Options
Upright Microscope System — For in vivo and in vitro multiphoton microscopy
The large stage clearance and long focus stroke of the standard upright frame accommodate a wide range of specimens, from tissue slices to live mice and other small animals.
Gantry Microscope System — For in vivo observations that require more space
The 355 mm height clearance between the objective and the base plate facilitates in vivo observations that require large apparatus, such as behavioral imaging in awake mice.
Inverted Microscope System — For in vitro observation of 3D cell (spheroid) and tissue cultures
The inverted frame provides a stable platform for time-lapse imaging of thick living specimens, especially tissue cultures and 3D spheroid and organoid cell cultures. This configuration is also useful for intravital imaging of organs and tissues through a body window in a small animal.
Applied Technology
Maximize Resolution in Deep Imaging
Get better resolution and contrast during deep imaging within thick specimens with TruResolution objectives.
These objectives have an automated correction collar that dynamically compensates for spherical aberration while maintaining accurate focus position. They automatically adjust at every plane of a volume image, delivering sharper and brighter 3D images at depth.
User-Friendly, Accurate Imaging with Automated Laser Alignment
Laser drift caused by wavelength tuning, temperature fluctuation, and other sources of cavity shift can cause a misalignment of the excitation beam in typical systems.
The FVMPE-RS multiphoton microscope maintains precise 4-axis laser alignment of the excitation beam into the scanner unit, even in the face of laser drift, simplifying system upkeep. The beam position and angle are automatically adjusted to deliver higher laser power and consistent pixel registration.
If your system has two excitation laser lines, this feature maintains co-alignment between the beams, helping eliminate co-registration errors between channels.
High-Speed Scanning at up to 438 Frames-per-Second
The fast resonant scanner and linear galvanometer scanner provide high-speed and high-resolution imaging in a single system. With capture rates of up to 438 fps at 512 × 32 pixels or 30 fps at 512 × 512 pixels across the full field of view (FN 18), you can:
- Avoid motion artifacts when imaging dynamic samples
- Track fast-moving cells in blood flow
- Observe rapid membrane potential dynamics across neurons and other cells