Overview

Accurate Cell Counting

  • Accurately count clustered cells: The counter identifies single, clustered, live, and dead cells for counts with an accuracy similar to a hemocytometer.
  • Exclude undesired particles with cell-size-based gates: Set the maximum and minimum cell sizes to filter for a given cell type.
  • Accurately count small cells: The Live Cell Sensitivity function optimizes the recognition rate for cells less than 8 μm.
  • Automated dilution calculator: Enter your desired concentrations, and the volume of cell suspension needed is automatically calculated.
  • No trypan blue staining required: The Cell Counter can quickly count and present the total number of cells in a sample that has not been treated with trypan blue. The number of live cells can be estimated when the proportion of live/dead cells in the sample is known.
  • Trypan blue concentration correction: The Auto Exposure Mode automatically corrects for variations in trypan blue concentration for stable count results.

Clear Cell Images with Liquid Lens Autofocusing

The Cell Counter model R1 automatically focuses on cells using liquid lens technology, which works similar to how human eyes focus. The lens can autofocus in the Z-axis at the right position, producing more accurate and reproducible cell counting results.

Efficient Cell Culture Data Management

Easy Data File Sharing

  • Store up to 1,000 counts
  • Easily export your data as a CSV file
  • Result and image data can be stored as a TIFF, an annotated TIFF, or a PDF report file
  • Easily transfer data using the cell counter’s USB ports

Comprehensive Report Results at a Glance

  • No need to manually record detailed settings for each count
  • Easily obtain report files containing the protocol used, the cell count results, the corresponding raw/analyzed cell images, and histograms
  • Use review mode to view various reports on the touch screen at the same time, including the number of live/dead cells and average cell size

Custom Cell Counting Parameters

Each detection parameter can be modified to fit the sample conditions. Up to 300 individual cell counting protocols can be saved and reloaded for use in routine procedures.

User-Friendly Cell Counter Design

Portable with a Small Footprint

The handle on top of the counter makes it easy to transport, while its small footprint enables it to fit in tight spaces in your lab. A storage space in the back of the counter hides the AC adaptor and extension cord.

Convenient Touch Screen

The large touch screen at the base of the cell counter offers stability while making it simple to count cells, view data, and print the results.

Easy-to-Load Slides

It’s easy to add cells to the cell counter’s disposable slides through the loading slits. Two sets of cell counting are performed on one slide, making it easy to replicate runs.

Specifications

Cell Counter model R1

Cell Counting Time *1 Less than 10 s (manual focusing)
Less than 15 s (auto focusing)
Cell Concentration Range 5 × 104–1 × 107 cells/mL
Cell Diameter Range 3–60 μm (recommended: 8–30 μm)
Output Information *2 Total/live/dead cell concentration
Total/live/dead cell number
Viability
Average cell size
Image Resolution 5 megapixels, color
Focusing Mode Automatic/manual focusing with liquid lens
Adjustable Parameters Size, roundness, dilution factor, noise reduction, declustering
Image Type TIFF, annotated TIFF
Report Format PDF
Count Data Storage 1,000 counts
Protocol Storage 300 protocols
Display LCD touchscreen: 800 × 480, 17.8 cm (7 in.)
USB Port 3 ports
Thermal Printer Built in
Dimensions (W × D × H) 195 mm × 237 mm × 272 mm (7.7 in. × 9.3 in. × 10.7 in.)
Weight 2.1 kg (4.6 lb) (without the external power adaptor)
Input Power AC 100–240 V, 50/60 Hz, 1.2 A

*1: Cell counting at less than 1 × 106 cells/ml concentration of HeLa or HL-60 cells.
*2: Live/dead cell concentration, number, and cell viability can be available with trypan blue mode.

Counting Slides

Material Polystyrene
Dimensions (W × D × H) 75 mm × 25 mm × 2.3 mm (3 in. × 1 in. × 0.09 in.)
Chamber Volume 10 μL
Quantity Per Box 100 counts (50 slides, 2 chambers per slide)

Resources

Application Notes
White Papers